Uvavanyo lokuQala olutsha oluphathwa ngesandla lwe-Covid-19 lwe-Nucleic Acid

Kutshanje, iPluslife Biotech, ishishini elizinze kwiGreater Bay Area, liphehlelele uVavanyo lokuqala oluphathwa ngesandla lweCovid-19 Nucleic Acid ukunceda kuthintelo nolawulo lobhubhane.         

IHong Kong ibethwe kanobom liza lamva nje losulelo lwe-COVID-19 kwezi veki zidlulileyo. Ukuphendula kwimingeni ebandakanya ukunqongophala kwabasebenzi kuthintelo kunye nolawulo lobhubhane, kunye nomthamo olinganiselweyo wovavanyo, urhulumente waseHong Kong SAR uye wakhupha inkqubo yendalo yonke, ehambisa izixhobo zovavanyo kunye nokuvumela abahlali ukuba bazivavanyele i-COVID-19. Abo bafuna ukuqinisekisa iziphumo banokuya kwizikhululo zovavanyo ukuze bafumane iimvavanyo ezongezelelweyo eziqinisekisayo ze-qPCR.

I-Dignosis and Treatment Protocol ye-COVID-19 (Trial Version 8), ekhutshwe ngokudibeneyo yiKomishoni yezeMpilo yeSizwe kunye noLawulo lweSizwe loNyango lwesiNtu lwaseTshayina, ichaza ngokucacileyo ukuba iziphumo ezilungileyo zovavanyo lwe-nucleic acid ngowona mgaqo uphambili wokuxilongwa kwe-COVID-19. Xa kuthelekiswa novavanyo olukhawulezayo lwe-antigen, ubuntununtunu kunye nokuchaneka kovavanyo lwe-nucleic acid luphezulu kakhulu, kwaye lunokubona abaguli abosulelekileyo kwinqanaba langaphambili. Nangona kunjalo, uvavanyo lwangoku lwe-nucleic acid oluqhelekileyo olufana novavanyo lwe-qPCR lufuna izixhobo ezibiza kakhulu kunye neenkqubo zokusebenza ezinzima, ngoko ke uvavanyo lusetyenziswa ikakhulu ezibhedlele, kumaziko ovavanyo lomntu wesithathu okanye iilabhoratri. Kanjalo, uvavanyo lwe-qPCR aluncedi ukufumana iziphumo zovavanyo ngokukhawuleza kwiindawo zovavanyo ezisekwe kuluntu.

Nangona uvavanyo lwe-antigen olukhawulezayo lulula kwaye kulula ukulusebenzisa, uvakalelo lwayo lusezantsi kakhulu kunolo lovavanyo lwe-qPCR. Kwiimeko ezininzi, inokubona kuphela iisampulu ezinamandla ezinamandla, kwaye ukuba ukuxinwa kweentsholongwane eziqulethwe kwiisampuli akufikeleli kwinani elithile, kuya kubakho amathuba okuba nee-negatives zobuxoki. Ke, kumanqanaba okuqala osulelo, uvavanyo lwe-qPCR luchaneke ngakumbi kunovavanyo lwe-antigen.

Unjingalwazi uZhou SONGYANG, uMseki wePluslife Biotech, uthe, “Ngokusasazeka ngokukhawuleza kwe-COVID-19 kunye nesidingo esonyukayo sokujongwa kwe-COVID-19, ukuphuhlisa iimveliso ezifanelekileyo ze-nucleic acid Point of Care Testing (POCT) ezinexabiso eliphantsi kwaye ezifanelekileyo njenge. Uvavanyo olukhawulezayo lwe-antigen, ngelixa ugcina ukuchaneka kunye nobuntununtunu obufana novavanyo lwe-qPCR, kunokuba nentsingiselo kuluntu luphela ukulawula ngcono ubhubhane. ”

I-Pluslife Biotech ngumphuhlisi kunye nomvelisi wovavanyo lwe-POCT nucleic acid kunye neemveliso zovavanyo lwasekhaya ezisekelwe kwi-Greater Bay Area. Inkampani ithumele ngokukhawuleza ibhetshi yokuqala yamawaka eekhithi zovavanyo ukuze zisetyenziswe kwizibhedlele zaseHong Kong kunye neekliniki. I-Pluslife Biotech ingamandla amatsha kumlo ochasene nobhubhane ngetekhnoloji, kwaye yenye yeenkampani zokuqala e-China ukuphehlelela uvavanyo lwe-in vitro diagnostic (IVD) olusekwe kwikhaya lwePOCT nucleic acid. I-Pluslife Mini Dock, ephuhliswe yi-Pluslife Biotech, yimveliso yokuqala yokuvavanywa kwe-POCT ye-nucleic acid kwi-COVID-19. Inkampani igqibe inkqubo yolawulo lomgangatho we-ISO13485 kunye nesatifikethi se-CE kwaye iphumelele intengiso kwimimandla emininzi kwihlabathi liphela.

Uvavanyo lwe-POCT ye-nucleic acid ye-Pluslife ifikelela kwinqanaba eliphezulu kakhulu lovakalelo, ukuchaneka kunye nokuchaneka, okufana novavanyo lwe-qPCR. Ikwakwazi ukubona ngokuzinzileyo iintsholongwane kwi-LoD ephantsi kakhulu (uMda wokuFumana). I-LoD yokwenene ezinzileyo ziikopi ezingama-200/mL, eyona ingcono kunovavanyo lwe-qPCR.

Ngaphezu koko, i-Pluslife Mini Dock ijongana neengxaki ezikhoyo zokuxhomekeka kwizixhobo ezibiza kakhulu (iyunithi enye idla ngokuxabisa ngaphezu kwamakhulu amawaka eedola zaseHong Kong), kwaye inokufikelela kuvavanyo lwe-nucleic acid kwindawo esezantsi kwaye ifumane iziphumo zovavanyo. ngoko nangoko. Ngokwendlela yokuvavanya, emva kokuthatha isampuli yangaphambili ye-nasal swab, abasebenzisi babeka nje i-swab kwi-lysate kunye nekhadi lokuvavanya, kwaye bafake ikhadi lokuvavanya kwi-Mini Dock yokuvavanya inyathelo elinye kwaye bafumane iziphumo.

Ngokumalunga novavanyo olusebenzayo, i-Pluslife Mini Dock inokubona isampula elungileyo malunga nemizuzu eyi-15 kwaye iqinisekise isampula engalunganga kwimizuzu engama-35, inciphisa kakhulu ixesha lokulinda xa kuthelekiswa novavanyo lwe-qPCR (ngokuqhelekileyo iiyure ezi-3-4 ngokutsalwa, kungabandakanywa ukuhanjiswa kwesampulu. ixesha lokuya kwilebhu). Ngokweendleko, ixabiso le-Pluslife Mini Dock lifikeleleka kakhulu kunezinye izixhobo zokuvavanya i-POCT nucleic acid kwimarike, kwaye iphinda isetyenziswe, iyenze ilungele ukusetyenziswa okukhulu kwinqanaba elisezantsi.

Ukuphumelela kweemveliso zePluslife Biotech ezinovakalelo kakhulu, ezinexabiso eliphantsi, ekulula ukuzisebenzisa nezithembekileyo zixhaswa liqela elinamandla okuvelisa izinto ezintsha kunye nokugxila okuzinikeleyo kubuchwephesha.

Unjingalwazi uZhou SONGYANG unamashumi eminyaka yobungcali kuphando kunye nophuhliso kwicandelo lobunjineli beprotheyini kunye nezinye iindawo zesayensi yobomi. Upapashe ngaphezu kwamanqaku e-150 njengombhali wokuqala kunye nombhali ohambelanayo kwiijenali ezaziwayo kumazwe ngamazwe ezifana neCell, iNdalo kunye neSayensi, ecatshulwa ngamaxesha angaphezu kwe-19,000. Iqela le-R & D lale nkampani libandakanya ibhetshi yokuqala yeetalente eziphezulu zaphesheya, oonjingalwazi, iiPhD kunye neengcali eziphezulu kwishishini le-IVD, abanamava amaninzi kwiiprotheni ezingundoqo, ubugcisa bokuvavanya, isakhiwo semveliso kunye nemveliso ezinzileyo.

Iimveliso zeQPCR zemveli zixhomekeke kumaqondo obushushu aphakamileyo kwaye zineemfuno eziphezulu zehardware, ezikhokelela kwiindleko eziphezulu zizonke zezixhobo; ngelixa uninzi lovavanyo olukhoyo lwe-isothermal nucleic acid lunokujongana nomba weendleko kwaye lube nesantya sokukhulisa ngokukhawuleza, kodwa alukwazi ukufikelela ngokuzinzileyo kubuntununtunu obulungileyo kunye nokuchaneka, okubenza bangakwazi ukulinganisa ngokuthe ngqo kwi-qPCR, ngoko ixesha elide bekungekho. uvavanyo lwe-POCT lwe-nucleic acid olwakhiwe kakuhle olunokuthi lusetyenziswe kwiintsapho kunye nakwiikliniki zempilo zoluntu.

Ukuphuhlisa imveliso yokuvavanya i-POCT ye-nucleic acid ephezulu, i-Pluslife Biotech iphuhlise i-RHAM, itekhnoloji esisiseko enamalungelo azimeleyo epropathi enomgangatho ophezulu wokuqonda, eyahlukileyo kubuchwepheshe bemveli bokukhulisa isothermal njenge-LAMP okanye i-CRISPR yokufumanisa itekhnoloji.

Itekhnoloji ye-RHAM ibonisa ukusebenza okufanayo njenge-qPCR, kwaye ingcono kakhulu kuneteknoloji yendabuko ye-isothermal amplification (efana ne-LAMP) ngokobuzwe, ukuzinza kunye neenkcukacha. Unyamezelo olubanzi kunye nokuhambelana okungcono kwe-RHAM kuqonda inyathelo elinye lokusetyenzwa kwesampulu, ukukhulisa kunye nokufumanisa konke-kwinye. Le nkqubo ayibandakanyi izenzo ezifana nokuvula isiciko emva kokukhulisa (akukho ngcoliseko ye-aerosol), kwaye ineemfuno eziphantsi zommandla wangaphandle kunye nenkxaso ye-hardware. Okwangoku, iPluslife Biotech yenze isicelo samalungelo awodwa omenzi wechiza angaphezu kwama-60 malunga neetekhnoloji ezahlukeneyo ezibandakanya i-RHAM, uninzi lwazo zinikezelwe.

Iimveliso zovavanyo lwe-COVID-19, ezimelwe yiPluslife Mini Dock, zivule amathuba angakumbi ovavanyo lwe-POCT nucleic acid. NgokukaNjingalwazi uZhou SONGYANG, i-Pluslife Mini Dock ingasetyenziswa kwiimeko ezinje ngezithethe, iindawo zovavanyo lwesikhululo seenqwelomoya, iimeko ezingxamisekileyo esibhedlele, uvavanyo lwangaphambi kotyando olukhawulezileyo, iilebhu ezihambahambayo / zasendle / uvavanyo kunye nomkhosi, iikliniki zoluntu, kunye nokuzivavanya ekhaya. Ngovavanyo olubhetyebhetye ngakumbi kwindawo, uthintelo lwendyikityha kunye nolawulo lunokufezekiswa kwindawo. Izigulana ze-COVID-19 nazo zinokubonwa kwaye zibekwe zodwa kwangethuba, ngelixa kuncitshiswa ixesha lokulinda kwabo baneziphumo ezibi.